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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
IFN-beta ELISA
IFN-beta ELISA from R&D Systems, a Bio-Techne Brand
Be the first to write a review!
Citations:
(46)
Supplier Page
Supplier Page from
R&D Systems, a Bio-Techne Brand for
IFN-beta ELISA
Request Info
Product Specs
Company Info
Citations
Reviews
Bench Tips
Product Specs
Item
IFN-beta ELISA
Company
R&D Systems, a Bio-Techne Brand
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
42400-1
Reactivity
Mouse
Detection Target
IFN-beta
Sensitivity
15.5 pg/mL
Detection Range
15.60 - 1000 pg/mL
NCBI Full Gene Name
interferon beta 1, fibroblast
NCBI Gene Aliases
IFN-beta, IFNB, If1da1, Ifb
Applications
ELISA
Brand/Trademark
R&D Systems, a Bio-Techne Brand
Assay Time
3.25 hours
Sample Type
Cell Culture Supernates (100 uL), Serum (100 uL)
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date
Quantity
1 Kit (also 1 Pack (5 Plates))
Specifications/Features
Available conjugates:
Biotin
Specificity:
Mouse IFN-beta
Add to Compare List
R&D Systems, a Bio-Techne Brand
614 McKinley Place N.E.
Minneapolis
,
Minnesota
55413
United States
Phone
: 1 (800) 343-7475
1 (612) 379-2956
Fax: 1 (612) 656-4400
Company Profile
Website:
http://www.rndsystems.com
Citations (46)
(1)
The Ability of Postimmunobiotics from L. rhamnosus CRL1505 to Protect against Respiratory Syncytial Virus and Pneumococcal Super-Infection Is a Strain-Dependent Characteristic
Microorganisms
November 3, 2022
Raya Tonetti F, Clua P, Fukuyama K, Marcial G, Sacur J, Marranzino G, Tomokiyo M, Vizoso-Pinto G, Garcia-Cancino A, Kurata S,
et al
.
(2)
Monitoring Cellular Immune Responses after Consumption of Selected Probiotics in Immunocompromised Mice
Food Science of Animal Resources
September 1, 2022
Seok-Jin Kang, Jun Yang, Na-Young Lee, Chang-Hee Lee, In-Byung Park, Si-Won Park, Hyeon Jeong Lee, Hae-Won Park, Hyun Sun Yun, Taehoon Chun
PBS) was also used to treat BMDMs. After
co-culture
, IFN-β (
R&D Systems
, Minneapolis, MN, USA, cat. #
42400-1
), IL-1β (Thermo Fisher Scientific, Waltham, MA, USA, cat. #
BMS6002
),
IL-6
(
Thermo Fisher Scientific
,
(3)
Camptothesome elicits immunogenic cell death to boost colorectal cancer immune checkpoint blockade
Journal of Controlled Release
September 1, 2022
Zhiren Wang, Wenpan Li, Jonghan Park, Karina Marie Gonzalez, Aaron James Scott, Jianqin Lu
manufacturer’s instructions of TNF-α (
Invitrogen
, #
KHC3011
),
IL-6
(
Abcam
,
ab222503
), IFN-β (
Bio-techne
, #
42400-1
) and IFN-γ (
LSBio
,
LS-F3414
) ELISA kits.
Western blotting The
tumor
tissues were homogenized by bead
(4)
An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP
The Journal of clinical investigation
February 1, 2022
Li X, Zhu Y, Zhang X, An X, Weng M, Shi J, Wang S, Liu C, Luo S, Zheng T.
24 hours. The IFN-β in the supernatants of splenocytes was detected with the mouse IFN-β ELISA Kit (
42400-1
,
R&D Systems
), following the manufacturer’s protocols.
Western blot analysis
. Total proteins were
(5)
Tumor cell intrinsic RON signaling suppresses innate immune responses in breast cancer through inhibition of IRAK4 signaling.
Cancer Letters
April 1, 2021
Jennifer R. Bourn, Sasha J. Ruiz-Torres, Brian G. Hunt, Nancy M. Benight, Susan E. Waltz
following the manufacturers protocol for the murine IFNα and IFNβ VeriKine ELISA kits (#
42120-1
and #
42400-1
;
R&D Systems, Inc
., Minneapolis, MN, USA). IFNα and IFNβ concentration (pg/ml) was measured at an optical
(6)
Small-Molecule Inhibitor of 8-Oxoguanine DNA Glycosylase 1 Regulates Inflammatory Responses during
Pseudomonas aeruginosa
Infection
The Journal of Immunology
October 15, 2020
Shugang Qin et al.
control), followed by PA14
infection
for 2 h. The expression level of IFNβ was determined by
ELISA
(
42400–1
,
R&D Systems
) according to the manufacturer’s instructions. (F)
Mitochondria
DNA deletion and replenishment
(7)
TMEM173 Drives Lethal Coagulation in Sepsis
Cell host & microbe
April 8, 2020
Zhang H, Zeng L, Xie M, Liu J, Zhou B, Wu R, Cao L, Kroemer G, Wang H, Billiar TR,
et al
.
Assay
Commercially available ELISA kits were used to
measure
the concentrations or activity of IFNβ (
DIFNB0
or
42400–1
,
R&D Systems
), F3 (
DCF300
,
R&D Systems
;
ab214091
,
Abcam
), fibrin (
MBS265263
or
MBS706338
,
MyBioSource
),
(8)
Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses
Communications biology
April 3, 2020
Oikawa D, Sato Y, Ohtake F, Komakura K, Hanada K, Sugawara K, Terawaki S, Mizukami Y, Phuong HT, Iio K,
et al
.
IL-6 DuoSet ELISA Kit (
R&D Systems
,
DY206-05
),
IFN beta Mouse ELISA kit
(
Thermo Fisher Scientific
,
424001
), Mouse CXCL10/IP-10/CRG-2 ELISA Kit (
Novus
,
NBP1-92665
). The open reading frames of
human
cDNAs14,19
(9)
MicroRNA-129-5p alleviates spinal cord injury in mice via suppressing the apoptosis and inflammatory response through HMGB1/TLR4/NF-κB pathway
Bioscience reports
March 27, 2020
Wan G, An Y, Tao J, Wang Y, Zhou Q, Yang R, Liang Q.
to quantify fluorescent
staining
.
ELISA
The levels of IFN-α (cat. no.
42120-1
),
Mouse
IL-1β (cat. no.
42400-1
), IL-6 (cat. no.
D6050
) and IL-10 (cat. no.
DY417
) in
mice
serum and cell supernatants were measured
(10)
Nickel induces inflammatory activation via NF-κB, MAPKs, IRF3 and NLRP3 inflammasome signaling pathways in macrophages
Aging (Albany NY)
December 10, 2019
Hongrui Guo et al.
were purchased from
Adipogen
. IL-1β (
MLB00C
), IL-6 (
M6000B
),
IL-18
(
7625
), TNF-α (
MTA00B
) and IFN-β (
42400-1
) ELISA kits were purchased from
R&D system
, while
IL-8
(
MBS261967
) ELISA kit was obtained from
MyBioSource
.
Cell isolation
(11)
The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses
Cell reports
May 14, 2019
Wang K, Ma H, Liu H, Ye W, Li Z, Cheng L, Zhang L, Lei Y, Shen L, Zhang F.
(12)
Small Heterodimer Partner Controls the Virus-Mediated Antiviral Immune Response by Targeting CREB-Binding Protein in the Nucleus
Cell reports
May 14, 2019
Kim JH, Yoon JE, Nikapitiya C, Kim TH, Uddin MB, Lee HC, Kim YH, Hwang JH, Chathuranga K, Chathuranga WAG,
et al
.
(13)
Initial Influenza Virus Replication Can Be Limited in Allergic Asthma Through Rapid Induction of Type III Interferons in Respiratory Epithelium
Frontiers in Immunology
May 17, 2018
Sujin An, Yung Jin Jeon, Ara Jo, Hyun Jung Lim, Young Eun Han, Sung Woo Cho, Hye Young Kim, Hyun Jik Kim
between samples. Quantification of Secreted Cytokines The levels of secreted IFN-α (
42120-1
), IFN-β (
42400-1
), IFN-λ2/3 (
DY1789B
), and IFN-γ (
DY485
) were quantified using a Duoset ELISA kit (
R&D Systems
; Minneapolis,
(14)
A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion
Immunity
April 17, 2018
Xia P, Wang S, Ye B, Du Y, Li C, Xiong Z, Qu Y, Fan Z.
(15)
The IKK-related kinase TBK1 activates mTORC1 directly in response to growth factors and innate immune agonists
The EMBO journal
January 4, 2018
Bodur C, Kazyken D, Huang K, Ekim Ustunel B, Siroky KA, Tooley AS, Gonzalez IE, Foley DH, Acosta-Jaquez HA, Barnes TM,
et al
.
h). Medium was collected to quantify IFN-b secretion using the
mouse
IFN-b ELISA Kit (
R&D Systems
;
42400-1
) according to manufacturer’s directions.
Protein assays
were used to normalized
cell number
per well
(16)
cGAS drives noncanonical-inflammasome activation in age-related macular degeneration
Nature medicine
January 1, 2018
Kerur N, Fukuda S, Banerjee D, Kim Y, Fu D, Apicella I, Varshney A, Yasuma R, Fowler BJ, Baghdasaryan E,
et al
.
mouse
interferon-β and
IL-18
in the media were detected using ELISA kits (
mouse
IFN-β,
R&D Systems
Cat#
42400-1
; mouse IL-18,
R&D Systems
Cat#
7625
; human IFN-β, R&D Systems Cat# 41410; human IL-18,
R&D systems
Cat#
(17)
The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity
Scientific reports
March 7, 2017
Doiron K, Goyon V, Coyaud E, Rajapakse S, Raught B, McBride HM.
requirements. Reagents ELISA kits for IL6 (
DY406
) and RANTES (
DY478
) were from
R&D systems
and Verikine IFNβ (
42400-1
) from
PBL
; anti-
MAPL
(
HPA017681
), anti-β-actin (
A2228
), anti-FLAG (
A8592
) and anti-vinculin (
V4505
)
(18)
Comparison of the Anti-Inflammatory Activities of Supercritical Carbon Dioxide versus Ethanol Extracts from Leaves of Perilla frutescens Britt. Radiation Mutant
Molecules (Basel, Switzerland)
February 17, 2017
Jin CH, Park HC, So Y, Nam B, Han SN, Kim JB.
(19)
The ubiquitin E3 ligase TRIM31 promotes aggregation and activation of the signaling adaptor MAVS through Lys63-linked polyubiquitination
Nature immunology
February 1, 2017
Liu B, Zhang M, Chu H, Zhang H, Wu H, Song G, Wang P, Zhao K, Hou J, Wang X,
et al
.
(20)
Rosmarinic Acid Methyl Ester Inhibits LPS-Induced NO Production via Suppression of MyD88- Dependent and -Independent Pathways and Induction of HO-1 in RAW 264.7 Cells
Molecules (Basel, Switzerland)
August 18, 2016
So Y, Lee SY, Han AR, Kim JB, Jeong HG, Jin CH.
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Reviews
Be the First to Write a Review!
Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
IFN-beta ELISA from R&D Systems, a Bio-Techne Brand
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Bench Tips
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